By Sasina Promdee
Year 2023
Abstract
This research aimed to: 1) investigate an appropriate carbon, nitrogen, and phosphorus source, 2) optimize the condition of PHA recovery using silica gel, 3) study the fabrication process of microcarriers by emulsion technique and 4) study the use of microcarriers in dynamic animal cell culture.
Novosphingobium sp. THA_AIK7 was cultured in a mineral salt medium (MSM) with various carbon, nitrogen, and phosphorus sources. Factor effecting PHAs extraction included temperature, loaded cell, and the amount of silica gel were analyzed using BBD. Fabrication of microcarrier from PHAs was designed using independent variables included PHA (mg), PVA (%), NH5CO3 (%), and stirrer (rpm). Fabricated microcarriers were tested for Vero and L929 cells culture.
Crude glycerol, MSG, and Na2HPO4·7H2O were the best carbon, nitrogen, and phosphorus sources for growth and PHA production with a maximum PHA content of 27.54%. The highest HV monomer derived from crude glycerol was at 10.85 mol%. The extracted yield of PHAs from silica gel was 41 mg, accounting for 51.25% of CDW. The average diameter and average pore size of fabricated microcarriers MC-PHAs were 125.864 μm and 0.970 μm, respectively. The viability of Vero and L929 cells were 92% and 100%, respectively. PHAs can be fabricated into microcarriers and are not toxic to animal cells. However, the properties of MC-PHAs would be improved to enhance cell adhesion and proliferation in the next step.